Synthesis and Characterization of Mn₃O₄ Nanoparticles for Biological Studies

Murugan Perachiselvi¹, Muthiah Sakthi Bagavathy¹, J. Jenson Samraj¹, E. Pushpalaksmi¹ and G. Annadurai^1,

¹Division of Nanoscience, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627412, India

Cite this paper:
Murugan Perachiselvi, Muthiah Sakthi Bagavathy, J. Jenson Samraj, E. Pushpalaksmi and G. Annadurai. Synthesis and Characterization of Mn₃O₄ Nanoparticles for Biological Studies. Applied Ecology and Environmental Sciences. 2020; 8(5):273-277. doi: 10.12691/aees-8-5-13

Abstract

Nowadays nanoparticles comprising the diversity of applications have been synthesized and used up in various fields. In our research, Manganese tetroxide NPs (Mn₃O₄) was synthesized by precipitation method. In the present investigation, antibacterial activity, as well as In-vitro cytotoxic effects of Mn₃O₄ NPs, have been evaluated. The cytotoxicity studies on the Vero cell line (African green monkey kidney cell line) were studied by using MTT assay at 72 hrs. The Cell viability which was observed during the process depends upon the time exposure and concentration. The antibacterial activity was evaluated against the bacteria such as Bacillus species, Escherichia coli, and Enterobacter sp. The synthesized nanoparticles were characterized using X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), Dynamic Light Scattering (DLS), and Fourier Transform Infra-Red Spectroscopy (FTIR). Moreover, In-vitro cytotoxic effects and the antibacterial activity of Mn₃O₄ NPs showed a better result at the concentration of 50 µg/100µl in Vero cell and the Zone of inhibition was measured for Enterobacter. This proves Mn₃O₄ NPs as promising biocompatible material.

Keywords:
Mn₃O₄ NPs cytotoxicity effects Vero cell and antibacterial activity

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References:

[1]	Ahmed, K.A.M. and Huang, K., “Formation of Mn3O4 nanobelts through the solvothermal process and their photocatalytic property,” Arabian Journal of Chemistry, 12 (3). 429-439. 2019.
[2]	Bello, A., Fashedemi, O.O., Lekitima, J.N., Fabiane, M., Dodoo-Arhin, D., Ozoemena, K.I., Gogotsi, Y., Charlie Johnson, A.T. and Manyala, N., “High-performance symmetric electrochemical capacitor based on graphene foam and nanostructured manganese oxide,” AIP Advances, 3 (8). 082118. 2013.
[3]	Chen, G., Roy, I., Yang, C. and Prasad, P.N., “Nanochemistry and nanomedicine for nanoparticle-based diagnostics and therapy,” Chemical Reviews, 116 (5). 2826-2885. 2016.
[4]	Chen, H. and He, J., “Facile synthesis of monodisperse manganese oxide nanostructures and their application in water treatment,” The Journal of Physical Chemistry C, 112 (45).17540 -17545. 2008.
[5]	Chen, J., Wu, X., Gong, Y., Wang, P., Li, W., Tan, Q. and Chen, Y., “Synthesis of Mn3O4/N-doped graphene hybrid and its improved electrochemical performance for lithium-ion batteries,” Ceramics International, 43(5). 4655-4662. 2017.
[6]	Dhaouadi, H., Ghodbane, O., Hosni, F. and Touati, F., “Mn3O4 Nanoparticles: Synthesis, Characterization, and Dielectric Properties,” ISRN Spectroscopy, 82-86. 2012.
[7]	Fang, M., Tan, X., Liu, M., Kang, S., Hu, X. and Zhang, L., “Low-temperature synthesis of Mn 3 O 4 hollow-tetrakaidecahedrons and their application in electrochemical capacitors,” CrystEngComm, 13(15).4915-4920. 2011.
[8]	Fernandes, C., Benfeito, S., Fonseca, A., Oliveira, C., Garrido, J., Garrido, E.M., and Borges, F., “Photodamage and photoprotection: toward safety and sustainability through nanotechnology solutions,” In Food Preservation (pp. 527-565). Academic Press. 2017.
[9]	Frewer, L.J., Gupta, N., George, S., Fischer, A.R.H., Giles, E.L., and Coles, D., “Consumer attitudes towards nanotechnologies applied to food production,” Trends in food science & technology, 40(2).211-225. 2014.
[10]	Fritsch, S., Sarrias, J., Rousset, A. and Kulkarni, G.U., “Low-temperature oxidation of Mn3O4 hausmannite,” Materials Research Bulletin, 33(8).1185-1194. 1998.
[11]	Hafez, A.A., Naserzadeh, P., Ashtari, K., Mortazavian, A.M. and Salimi, A., “Protection of manganese oxide nanoparticles-induced liver and kidney damage by vitamin D,” Regulatory Toxicology and Pharmacology, 98.240-244. 2018.
[12]	Jiménez-Pérez, Z.E., Singh, P., Kim, Y.J., Mathiyalagan, R., Kim, D.H., Lee, M.H. and Yang, D.C., “Applications of Panax ginseng leaves-mediated gold nanoparticles in cosmetics relation to antioxidant, moisture retention, and whitening effect on B16BL6 cells,” Journal of ginseng research, 42(3). 327-333. 2018.
[13]	Khan, S., Ansari, A.A., Khan, A.A., Abdulla, M., Al-Obeed, O. and Ahmad, R., “In vitro evaluation of anticancer and biological activities of synthesized manganese oxide nanoparticles,” MedChemComm, 7(8).1647-1653. 2016.
[14]	Khedkar, M., Michael, P.E. and Khan, S.R., “Copper and Copper Oxide Nanoparticles: Applications in Catalysis,” 2019.
[15]	Kumar, S., Kaur, R., Rajput, R. and Singh, M., “Bio-Pharmaceutics Classification System (BCS) Class IV Drug Nanoparticles: Quantum Leap to Improve Their Therapeutic Index,” Advanced pharmaceutical bulletin, 8(4).617.624. 2018.
[16]	Li, L., Seng, K.H., Liu, H., Nevirkovets, I.P. and Guo, Z., “Synthesis of Mn3O4-anchored graphene sheet nanocomposites via a facile, fast microwave hydrothermal method and their supercapacitive behavior.” Electrochimica Acta, 87, 801-808. 2013.
[17]	Li, W.N., Yuan, J., Shen, X.F., Gomez‐Mower, S., Xu, L.P., Sithambaram, S., Aindow, M. and Suib, S.L., “hydrothermal synthesis of structure‐and shape‐controlled manganese oxide octahedral molecular sieve nanomaterials,” Advanced Functional Materials, 16(9). 1247-1253. 2006.
[18]	Liu, M., Wang, Y., Cheng, Z., Zhang, M., Hu, M. and Li, J., “Electrospun Mn2O3 nanowrinkles and Mn3O4 nanorods: Morphology and catalytic application,” Applied Surface Science, 313.360-367. 2014.
[19]	Luo, J.D., Wang, Y.Y., Fu, W.L., Wu, J. and Chen, A.F., “Gene therapy of endothelial nitric oxide synthase and manganese superoxide dismutase restores delayed wound healing in type 1 diabetic mice,” Circulation, 110(16).2484-2493. 2004.
[20]	Markides, H., Rotherham, M. and El Haj, A.J., “Biocompatibility and toxicity of magnetic nanoparticles in regenerative medicine,” Journal of Nanomaterials, 8,213-216.2012.
[21]	Masdor, N.A., Altintas, Z. and Tothill, I.E., “Sensitive detection of Campylobacter jejuni using nanoparticles enhanced QCM senso,”. Biosensors and Bioelectronics, 78.328-336. 2016.
[22]	Mo, C.M., Li, Y.H., Liu, Y.S., Zhang, Y. and Zhang, L.D., “Enhancement effect of photoluminescence in assemblies of nano-ZnO particles/silica aerogels,” Journal of applied physics, 83(8).4389-4391. 1998.
[23]	Nicoloff, G., Blazhev, A., Petrova, C. and Christova, P., “Circulating immune complexes among diabetic children,” Journal of Immunology Research, 11(1).61-66. 2004.
[24]	Pi, J.K., Yang, H.C., Wan, L.S., Wu, J. and Xu, Z.K., “Polypropylene microfiltration membranes modified with TiO2 nanoparticles for surface wettability and antifouling property,” Journal of Membrane Science, 500.8-15.2016.
[25]	Raj, B.G.S., Asiri, A.M., Wu, J.J. and Anandan, S., “Synthesis of Mn3O4 nanoparticles via chemical precipitation approach for supercapacitor application,” Journal of Alloys and Compounds, 636.234-240.2015.
[26]	Ranjan, M., Bhatnagar, M. and Mukherjee, S., “Localized surface plasmon resonance anisotropy in template aligned silver nanoparticles: A case of biaxial metal optics,” Journal of Applied Physics, 117(10).103106.2015.
[27]	Reddy, R.N. and Reddy, R.G., “Sol-gel MnO2 as an electrode material for electrochemical capacitors,” Journal of Power Sources, 124(1).330-337.2003.
[28]	Shaik, D.P., Pitcheri, R., Qiu, Y. and Hussain, O.M., “Hydrothermally synthesized porous Mn3O4 nanoparticles with enhanced electrochemical performance for supercapacitors,” Ceramics International, 45(2).2226-2233.2019.
[29]	Shukla, S., Jadaun, A., Arora, V., Sinha, R.K., Biyani, N. and Jain, V.K., “In vitro toxicity assessment of chitosan oligosaccharide coated iron oxide nanoparticles,” Toxicology reports, 2. 27-39. 2015.
[30]	Tholkappiyan, R., Naveen, A.N., Vishista, K., and Hamed, F., “Investigation on the electrochemical performance of hausmannite Mn3O4 nanoparticles by ultrasonic irradiation assisted co-precipitation method for supercapacitor electrodes,” Journal of Taibah University for Science, 12 (5).669-677.2018.
[31]	Ullah, A.A., Kibria, A.F., Akter, M., Khan, M.N.I., Maksud, M.A., Jahan, R.A. and Firoz, S.H., “Synthesis of Mn3O4 nanoparticles via a facile gel formation route and study of their phase and structural transformation with distinct surface morphology upon heat treatment,” Journal of Saudi Chemical Society, 21(7).830-836. 2017.
[32]	Vélez, M.A., Perotti, M.C., Santiago, L., Gennaro, A.M. and Hynes, E., “Bioactive compounds delivery using nanotechnology: design and applications in dairy food In Nutrient delivery”, Academic Press, 221-250.2017.
[33]	Versiani, A.F., Andrade, L.M., Martins, E.M., Scalzo, S., Geraldo, J.M., Chaves, C.R., Ferreira, D.C., Ladeira, M., Guatimosim, S., Ladeira, L.O. and da Fonseca, F.G., “Gold nanoparticles and their applications in biomedicine,” Future Virology, 11(4). 293-309. 2016.