Applied Ecology and Environmental Sciences
ISSN (Print): 2328-3912 ISSN (Online): 2328-3920 Website: http://www.sciepub.com/journal/aees Editor-in-chief: Alejandro González Medina
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Applied Ecology and Environmental Sciences. 2020, 8(5), 229-232
DOI: 10.12691/aees-8-5-6
Open AccessArticle

Eco-Genotoxic Effects of Certain Pesticide Mixtures to Earthworm, Eisenia Fetida

B. Reddi Bhargavi1, Ravi Kumar Vellanki1, Rajanikanth Akurathi2 and Jagadeeswara Reddy Kanala2,

1Department of Biotechnology, Vignan University, Vadlamudi, Guntur-522213, AP, India

2Sugen Life Sciences Pvt.Ltd, Tirupati - 517505, AP, India

Pub. Date: July 02, 2020

Cite this paper:
B. Reddi Bhargavi, Ravi Kumar Vellanki, Rajanikanth Akurathi and Jagadeeswara Reddy Kanala. Eco-Genotoxic Effects of Certain Pesticide Mixtures to Earthworm, Eisenia Fetida. Applied Ecology and Environmental Sciences. 2020; 8(5):229-232. doi: 10.12691/aees-8-5-6

Abstract

In this study, Eco-Genotoxic effect of two commercially available pesticides mixtures, Deltamethrin 1% + Triazophos 35% EC (D+T) and Profenofos 40% EC + Cypermethrin 4% EC (P+C), being used widely in agriculture were evaluated on earthworm Eisenia fetida. Initially LC50 for each pesticide mixture was determined by conducting acute toxicity study followed by exposing earthworms to three lowest concentrations of pesticide mixtures for 72 hours. At the end of the exposure period, the coelomocytes were isolated from the earthworm and processed for comet assay. Acute toxicity test revealed that the toxic concentration of D+T (EC) and P+C (EC) at 0.6mg/ml and 0.5mg/ml of distilled water, shown LC50 value of mortality as compare to control set of experiment. The behavior of Earthworm has been changed like body folded and less active as per the increased the dose of the toxicants. On analysis it was observed that D+T (EC) induced strand breaks at a concentration of 0.3mg/ml, whereas P+C EC induced strand breaks at 0.3 and 0.6mg/ml. From the results it is concluded that the pesticide mixtures evaluated in the study could be moderately genotoxic to earthworm Eisenia fetida.

Keywords:
Eco-Genotoxicity LC50 comet assay pesticide mixtures Emulsifiable concentrate (EC)

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References:

[1]  Pawar, SS., and Ahmad Shahzad. (2014). Filter Paper Contact Test Method for Estimation of Toxic Effect of Chloropyriphose on Earthworm, Eisenia foetida. International research journal of science and engineering. 2(1): 23-25.
 
[2]  Aira, M., Monroy, F., and Dominguez, J. (2006). C to N ratio strongly affects population structure of Eisenia fetida in vermicomposting systems. European Journal of Soil Biology. 42: S127-S131.
 
[3]  OECD. (1984). Organization for Economic Cooperation and Development. Guideline for Testing of Chemicals, No. 207, Earthworm Acute Toxicity. Paris, France.
 
[4]  Rishikesh K. Tiwari., Shikha Singh., Ravi S. Pandey. (2019). Assessment of acute toxicity and biochemical responses to chlorpyrifos, cypermethrin and their combination exposed earthworm, Eudrilus eugeniae. Toxicology Reports. 6: 288-297.
 
[5]  Laura Mincarelli, Costantino Vischetti, John Craft and Luca Tiano. (2016). DNA damage in different Eisenia andrei coelomocytes sub-populations after in vitro exposure to hydrogen peroxide. Springer Plus. 5:302.
 
[6]  Jha, A.N. (2008). Ecotoxicological applications and significance of the comet assay. Mutagenesis. 23(3): 207-221.
 
[7]  Rojas, E., Lopez, M.C., and Valverde, M. (1999). Single cell gel electrophoresis assay: Methodology and applications. Journal of Chromatography B: Biomedical Sciences and Applications.54: 722-225.
 
[8]  Singh, N.P., McCoy, M.T., Tice, R.R., and Schneider, E.L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research. 175: 184-191.
 
[9]  Eyambe, G.S., Goven, A.J., Fitzpatrick, L.C., Venables, B.J., and Cooper, E.L. (1991). A non-invasive technique for sequential collection of earthworm (Lumbricus terrestris) leukocytes during subchronic immunotoxicity studies. Laboratory Animals. 25: 61-67.
 
[10]  Collins, A.R., Dusinska, A., Franklin, M., Somorovska, M., Petrovska, H., Duthie, S., Fillion, L., Panayoitidis, M., Raslova, K., and Vaughan, N. (1997). Comet assay in human biomonitoring studies: reliability, validation and applications. Environmental Molecular Mutagenesis. 30:139-146.
 
[11]  Collins, A.R. (2002). The comet assay, principles, applications and limitations. In: Didenko VV (ed.) In Situ Detection of DNA Damage. Methods and Protocols, v. 203. Humana Press Inc., Totowa, 163-177.
 
[12]  Shikha Singh, Rishikesh K. Tiwari., Ravi S. Pandey. (2019). Acute toxicity evaluation of triazophos, deltamethrin and their combination on earthworm, Eudrilus eugeniae and its impact on AChE activity. Chemistry and ecology.1-13.
 
[13]  Jiqiang Chen, Muhammad Saleem, Caixia Wang, Wenxing Liang & Qingming Zhang. (2018). Individual and combined effects of herbicide tribenuron-methyl and fungicide tebuconazole on soil earthworm Eisenia fetida. Scientific Reports. 8:2967.